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It didn't take long to realise that Martine's reference to "being in the nod" meant she was actually naked, baring her shoulders to her 423k followers. Amid calls for social media reform, it has also cast a spotlight on Mr McNutt's life and struggles in the days and years leading up to his suicide. Who was Ronnie McNutt?

Dutta K, Dasu VV. Synthesis of short chain alkyl esters using cutinase from Burkholderia cepacia NRRL B2320. J Mol Catal B Enzym. 2011;72:150–6.That was when Mr Steen, through a podcast listener, found out that his friend was in need of immediate help.

The New Albany Police Department was alerted about a man with a gun threatening to take his own life on a Facebook live stream, and officers were sent to Mr McNutt's flat. Social media users were left horrified as a video circulated of a man tragically live-streaming his own death. The reason for the act he decided to do was supposed to be his dismissal from work and also the breakup with his girlfriend. The live broadcast was also noticed by Ronnie’s friend Joshua Steen, who tried to help him and called the police. Qin Z, Yan Q, Yang S, Jiang Z. Modulating the function of a β-1,3-glucanosyltransferase to that of an endo-β-1,3-glucanase by structure-based protein engineering. Appl Microbiol Biotechnol. 2016;100:1765–76. For scale-up cutinase production, the transformant showing the highest cutinase activity in shake-flask culture was cultivated in a 5-L fermentor with 1.5 L working volume at 30 °C. The fermentation process including glycerol batch phase, glycerol fed-batch phase, and methanol fed-batch phase was performed according to Pichia Fermentation Guidelines (Version B, 053002, Invitrogen Inc.). The fermentation medium for cutinase production in the fermentor was FBS medium containing 4.35 mL/L of PTM 1 trace salts. Throughout the cultivation period, the dissolved oxygen level was maintained above 20% by adjusting the air flow rate and agitation speed. The initial pH was controlled at 4.0 by ammonium hydroxide. Upon depletion of initial glycerol, fed-batch fermentation was initiated with the addition of 50% (w/v) glycerol containing 12 mL/L PTM 1 trace salts at a rate of 18.4 mL/h/L (initial medium volume). After a 6-h glycerol fed-batch phase, the methanol induction phase was started with the addition of 100% methanol containing 12 mL/L PTM 1 trace salts at a flow rate of 10 mL/h/L (initial medium volume), and the medium pH was adjusted to 6.0. During the methanol induction phase, samples were withdrawn every 12 h, and dcw, cutinase activity, and protein content were determined. The cell-surface cutinase activity on P. pastoris cell wall was measured by the method of Su et al. [ 48]. The cells were collected by centrifugation at 11, 510 g for 10 min, washed three times with distilled water, and resuspended in 50 mM Tris–HCl pH 8.0, and the activity of the suspended solution was then assayed according to the standard method. The dry cell weight (dcw) was quantified by drying the washed cells until a constant weight was achieved. Enzyme assay and protein determination

He added: "We have seen catastrophic breakdowns of terms of service and sets of standards from every single major social media platform over the past week. One friend wrote: “Please say a prayer right now for the family of Ronnie McNutt. He just killed himself live on FB and I cannot unsee this. Read More Related Articles Martine McCutcheon suffers from 'migraines from hell' amid fibromyalgia flare-ups". 13 August 2021. Archived from the original on 12 August 2021 . Retrieved 13 August 2021. Wakefield, Jane (September 19, 2020). "Friend challenges Facebook over Ronnie McNutt suicide video". www.bbcnews.com. BBC News . Retrieved March 25, 2022.

Mr McNutt, a former Army veteran who served in Iraq, worked at a Toyota plant in Blue Springs, New Albany. There are unconfirmed reports that he had lost his job and broken up with his girlfriend before his suicide. I just thought my health, money, career have all been taken from me, and now even my engagement ring - the one thing that represents what is solid in my life - has gone," she said in 2016. The campaign calls on social media networks to accept responsibility for "all content created, posted, and distributed" on their platforms, adding: "You must immediately stop passing the blame on to the users when content you distribute actively harms them. A piece of my mind: Martine McCutcheon". The Herald. 16 November 2009. Archived from the original on 17 March 2014 . Retrieved 24 November 2009.Xu H, Yan Q, Duan X, Yang S, Jiang Z. Characterization of an acidic cold-adapted cutinase from Thielavia terrestris and its application in flavor ester synthesis. Food Chem. 2015;188:439–45. McCutcheon released her autobiography, Who Does She Think She Is? in 2000, and in 2003 Martine McCutcheon: Behind the Scenes – A Personal Diary, was published by HarperCollins. McCutcheon was one of many celebrities who featured in a 2004 promotional film for London's successful bid for the 2012 Summer Olympics. A fitness DVD, Martine McCutcheon: Dance Body, was released in December 2005.

The 80/20 rule is what works for me," she continued. "For 80 per cent of my week, I will have mainly just healthy proteins, vegetables and fruit, organic and veggie juices, and Flora ProActiv spread. Roth C, Wei R, Oeser T, Then J, Föllner C, Zimmermann W, Sträter N. Structural and functional studies on a thermostable polyethylene terephthalate degrading hydrolase from Thermobifida fusca. Appl Microbiol Biotechnol. 2014;98:7815–23. Anguish of Martine's mum". Evening Standard. 18 April 2005. Archived from the original on 24 August 2017 . Retrieved 24 August 2017. Seman WMKW, Bakar SA, Bukhari NA, Gaspar SM, Othman R, Nathan S, Mahadi NM, Jahim J, Murad AMA, Bakar FDA. High level expression of Glomerella cingulata cutinase in dense cultures of Pichia pastoris grown under fed-batch conditions. J Biotechnol. 2014;184:219–28.We need social media reform immediately; any action other than immediate action is far too late. We need your help to bring about this immediate change." She wrote in the accompanying caption: "My heart is forever broken. My baby brother, my gentle giant, sadly passed away, suddenly, 2 weeks ago. He was 31 years old." Vile trolls have harassed Mr McNutt's family online, set up fake accounts under his name and claimed the suicide was faked, and created fake fundraising campaigns. Calls for social media reform Kitching, Chris (September 14, 2020). "Tragic story of Ronnie McNutt who killed himself on Facebook live stream". Mirror . Retrieved January 2, 2023.